Human Ubiquitin

Recombinant Human Ubiquitin (genbank accession number 229532), with a C-terminal extension (HS UBI-SHHHHHH), is expressed in E.coli using Celtone® growth medium. The protein is purified by non-denaturing methods to homogeneity as determined by SDS-PAGE. Purified protein is lyophilized under conditions preventing deleterious pH changes and packaged under vacuum. Performance is ensured for each batch by nuclear magnetic resonance (NMR) testing.

(Storage: Lyophilized protein in unopened containers may be stored for extended periods at -20^oC. Once solubilized, the protein should be stored at 4^oC and aseptic conditions should be maintained.)

¹H¹⁵N-HSQC NMR spectrum of uniformly ¹⁵N,¹³C,²H-labeled Ubiquitin



¹H¹⁵N-HSQC NMR spectrum of uniformly ¹⁵N,¹³C,²H-labeled Ubiquitin. Sample concentration was 0.7 mM in 50 mM phosphate buffer in 90% H₂O/10% D₂O at pH=6 with 1mM NaN₃. NMR measurements were performed at 30ºC on a 500 MHz system. The spectrum was recorded with 128 msec acquisition time in the proton dimension and 75 msec acquisition time in the nitrogen dimension. All characteristic cross-peaks were observed and chemical shifts are in excellent agreement with published literature data (Wang et al., “Journal of Biomolecular NMR.” 1995,
(376-382).

Description		% Isotope Enrichment		Product No.		Quantity		Price
Ubiquitin-U		natural		50UBH0		10 mg		Inquire
Ubiquitin-d		2H, >97%		51UBH98		10 mg		Inquire
Ubiquitin-C		13C, >98%		52UBH98		10 mg		$3,000
Ubiquitin-N		15N, >98%		53UBH98		10 mg		$1,100
Ubiquitin-CN		15N, >98%;13C, >98%		54UBH98		10 mg		$2,800
Ubiquitin-dN		15N, >98%; 2H, >97%		56UBH98		10 mg		$3,000
Ubiquitin-dCN		15N, >98% 13C, >98% 2H, >97%		57UBH98		10 mg		Inquire

Recommended Applications:
    This product is intended for use as an NMR standard.