Individual 2' Deoxyribonucleotide Phosphoramidites


Individual Deoxyribonucleoside Phosphoramidites are produced from the corresponding 5'-monohosphates by dephosphorylation and chemical synthesis. Each nucleoside is derivatized as the 5'-O-(4, 4'-dimethoxytrityl) 3'-O-(2-cyanoethyl)-N-diisopropylphosphoramidite. dA and dC residues are further protected by 6-N- and 4-N-benzoyl groups respectively. dG residues are further protected by 2-N-isobutyryl groups. The products are supplied as a powder under argon gas.

Description % Isotope Enrichment Product No.
 Quantity Price
dA-15N-Phosphoramidite   15N, >98%   536D3A98
   5 mg   $425
dC-15N-Phosphoramidite   15N, >98%   536D3C98   5 mg   $245
dG-15N-Phosphoramidite   15N, >98%  

536D3G98

   5 mg   $245
dT-15N-C   15N, >98%
  

536D3T98

   5 mg   $425
dA-13C, 15N -Phosphoramidite   15N, >98%;13C, >98%
  

546D3A98

   5 mg   $570
13C, 15N -Phosphoramidites available on request

NOTE: Using a conventional oligonucleotide synthesizer without any modification to the software, the amount of each phosphoramidite required is as follows:

0.2 umole scale: 3.3 mg per each base required as labeled
1.0 umole scale: 16.5 mg per each base required as labeled